FOXP3 Antikörper (CF®740)

Produktnummer ABIN7654220

Der Maus Monoklonal Anti-FOXP3-Antikörper wurde für FACS validiert. Er ist geeignet, FOXP3 in Proben von Human, Maus und Affe zu detektieren.

Kurzübersicht für FOXP3 Antikörper (CF®740) (ABIN7654220)

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reaktivität: Human, Maus, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FOXP3 Antikörper ist konjugiert mit CF®740

Applikation: Flow Cytometry (FACS)

Klon: 3G3

Produktdetails anti-FOXP3 Antikörper (CF®740)

Verwendungszweck: FOXP3(3G3), CF740 conjugate

Produktmerkmale: Recognizes a protein of 47-55 kDa, which is identified as FOXP3. Its precise epitope is not known, but it has been mapped to the N-terminal portion (amino acids 2-136) of the protein.The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. During early development, FOXP1 and FOXP2 are expressed abundantly in the lung, with lower levels of expression in neural, intestinal and cardiovascular tissues, where they act as transcription repressors. FOXP1 is widely expressed in adult tissues, while neoplastic cells often exhibit a dramatic change in expression level or localization of FOXP1. Mutations in FOXP3 gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Full-length His-tagged mouse FOXP3 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Tonsil, lymph node or Breast carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Andere Bezeichnung: FOXP3

Hintergrund:

Synonyms: JM2, AIID, DIETER, IPEX, MGC141961, MGC141963, PIDX, XPID, FOXP3delta7, forkhead box protein P3, immune dysregulation, polyendocrinopathy, enteropathy, X-linked, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, scurfin

Gene Symbol: FOXP3

Molekulargewicht: 47-55 kDa

Gen-ID: 50943

UniProt: Q9BZS1

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation