Filaggrin Antikörper (AA 998-1104) (CF®740)

Produktnummer ABIN7654084

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Filaggrin in IHC und IHC (fp). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Filaggrin Antikörper (AA 998-1104) (CF®740) (ABIN7654084)

Target: Filaggrin (FLG)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Filaggrin Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: FLG-1561

Produktdetails anti-Filaggrin Antikörper (CF®740)

Bindungsspezifität: AA 998-1104

Verwendungszweck: Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561), CF740 conjugate

Produktmerkmale: Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Filaggrin protein fragment (aa 998-1104)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer pH 6.0for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: U-251-MG cells or Skin

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Filaggrin

Target: Filaggrin (FLG)

Andere Bezeichnung: Filaggrin

Hintergrund:

Synonyms: ATOD2, Epidermal Filaggrin, Filaggrin, Filaggrin precursor, Fillagrin, FLG, Profilaggrin

Gene Symbol: FLG

Molekulargewicht: 26-45 kDa (Processed), 350 kDa (Profilaggrin)

Gen-ID: 2312

UniProt: P20930

Pathways: Sensory Perception of Sound, Stem Cell Maintenance