Fibronectin Antikörper (CF®740)

Produktnummer ABIN7654049

Der Maus Monoklonal Anti-Fibronectin-Antikörper wurde für WB, IHC, IF, ELISA, IHC (fp) und Func validiert. Er ist geeignet, Fibronectin in Proben von Human zu detektieren.

Kurzübersicht für Fibronectin Antikörper (CF®740) (ABIN7654049)

Target: Fibronectin

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Fibronectin Antikörper ist konjugiert mit CF®740

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Functional Studies (Func)

Klon: HFN7-1

Produktdetails anti-Fibronectin Antikörper (CF®740)

Verwendungszweck: Fibronectin(HFN7.1), CF740 conjugate

Produktmerkmale: Fibronectin is a dimeric glycoprotein of 440 kDa, which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. This MAb is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human fibronectin purified from serum by affinity chromatography on gelatin-sepharose

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: SW156 cells or Kidney

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Fibronectin

Target: Fibronectin

Hintergrund:

Synonyms: Cold insoluble globulin (CIG), FINC, FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor (MSF), Ugl-Y3

Gene Symbol: FN1

Molekulargewicht: 220 kDa (monomer), 440 kDa (dimer)

Gen-ID: 2335

UniProt: P02751