Fibronectin Antikörper (CF®740)

Produktnummer ABIN7654047

Dieses Anti-Fibronectin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Fibronectin in WB, IHC, FACS und IHC (fp). Geeignet für Human, Ratte, Maus und Schwein.

Kurzübersicht für Fibronectin Antikörper (CF®740) (ABIN7654047)

Target: Fibronectin

Reaktivität: Human, Ratte, Maus, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Fibronectin Antikörper ist konjugiert mit CF®740

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: TV-1

Produktdetails anti-Fibronectin Antikörper (CF®740)

Verwendungszweck: Fibronectin(TV-1), CF740 conjugate

Produktmerkmale: Fibronectin is a soluble dimeric glycoprotein of 440 kDa, which is present in cells, extracellular matrix, and blood. This MAb reacts with the cellular as well as plasma form of fibronectin. Reportedly, after iv administration, this MAb localizes to tumor vessels where it binds to the underlying basement. The epitope recognized by this antibody is not accessible to the circulating MAb in normal tissues, indicating that it can be used to specifically target tumor vessels in vivo. TV-1 is reportedly useful for delivering vasoactive agents to tumors to induce increased vascular permeability or blood flow prior to treatment with chemotherapeutic drugs or MAbs.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: T-cell lymphoma biopsy

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: SW156 cells or Kidney

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Fibronectin

Target: Fibronectin

Hintergrund:

Synonyms: Cold insoluble globulin (CIG), FINC, FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor (MSF), Ugl-Y3

Gene Symbol: FN1

Molekulargewicht: 220 kDa (monomer), 440 kDa (dimer)

Gen-ID: 2335

UniProt: P02751