FTL Antikörper (AA 38-165) (CF®740)

Produktnummer ABIN7654013

Der Maus Monoklonal Anti-FTL-Antikörper wurde für WB, IHC und IHC (fp) validiert. Er ist geeignet, FTL in Proben von Human zu detektieren.

Kurzübersicht für FTL Antikörper (AA 38-165) (CF®740) (ABIN7654013)

Target: FTL (Ferritin, Light Polypeptide (FTL))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FTL Antikörper ist konjugiert mit CF®740

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: FTL-1388

Produktdetails anti-FTL Antikörper (CF®740)

Bindungsspezifität: AA 38-165

Verwendungszweck: Ferritin, Light Chain (FTL) (Microglia Marker) (FTL/1388), CF740 conjugate

Produktmerkmale: Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human FTL protein fragment (aa 38-165)

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin) 0.1-0.2 μg/mL. Flow cytometry 0.1-0.2 μg/million cells Immunofluorescence 0.1-0.2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HepG2, HeLa, HL-60 or 293T cells. Pancreas, Liver, Cerebellum or Testis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu FTL

Target: FTL (Ferritin, Light Polypeptide (FTL))

Andere Bezeichnung: Ferritin, Light Chain

Hintergrund:

Synonyms: Ferritin L chain, Ferritin L subunit, Ferritin light chain, Ferritin light polypeptide, FTL, LFTD, NBIA3

Gene Symbol: FTL

Molekulargewicht: 19-25 kDa

Gen-ID: 2512

UniProt: P02792

Pathways: Transition Metal Ion Homeostasis