Fc gamma RII (CD32) Antikörper (CF®740)

Produktnummer ABIN7653982

Dieses Maus Monoklonal-Antikörper erkennt spezifisch in FACS, WB, IP und Func. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Fc gamma RII (CD32) Antikörper (CF®740) (ABIN7653982)

Target: Fc gamma RII (CD32)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: CF®740

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func)

Klon: 7-3

Produktdetails

Verwendungszweck: CD32(7.3), CF740 conjugate

Produktmerkmale: This MAb reacts with a CD32 (FcgRII) epitope (cluster-4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcgRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: K562 and FcgRII L cells

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: K-562 cells. Lymph node, tonsil and placenta.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Antigendetails

Target: Fc gamma RII (CD32)

Andere Bezeichnung: CD32

Hintergrund:

Synonyms: CD32A, Fc fragment of IgG low affinity IIa receptor, Fc gamma RIIa, Fc-gamma RII-a, Fc-gamma-RIIa, FCGR2A, FcgRII, Fcr-2, FcRII-a, IGFR2, IgG Fc receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-a, Ly-17, Ly-m20, LyM-1, Lymphocyte antigen 17

Gene Symbol: FCGR2A

Tissue Expression: Endothelial cells

Molekulargewicht: 40 kDa

Gen-ID: 2212

UniProt: P12318