FBXO5 Antikörper (AA 1-250) (CF®740)

Produktnummer ABIN7653910

Dieses Maus Monoklonal-Antikörper erkennt spezifisch FBXO5 in WB, IHC und IHC (fp). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für FBXO5 Antikörper (AA 1-250) (CF®740) (ABIN7653910)

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FBXO5 Antikörper ist konjugiert mit CF®740

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: EMI1-1176

Produktdetails anti-FBXO5 Antikörper (CF®740)

Bindungsspezifität: AA 1-250

Verwendungszweck: EMI1(EMI1/1176), CF740 conjugate

Produktmerkmale: This antibody recognizes a 56 kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Western blotting 1-2 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HeLa, 293 or HepG2 cells. Ovarian carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu FBXO5

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Andere Bezeichnung: EMI1

Hintergrund:

Synonyms: Early Mitotic Inhibitor-1 (EMI1), FBX5, Fbxo31

Gene Symbol: FBXO5

Molekulargewicht: 56 kDa

Gen-ID: 26271

UniProt: Q9UKT4

Pathways: Mitotic G1-G1/S Phases