EPX Antikörper (CF®740)

Produktnummer ABIN7653523

Dieses Maus Monoklonal-Antikörper erkennt spezifisch EPX in IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für EPX Antikörper (CF®740) (ABIN7653523)

Target: EPX (Eosinophil Peroxidase (EPX))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EPX Antikörper ist konjugiert mit CF®740

Applikation: Immunofluorescence (IF)

Klon: AHE-1

Produktdetails anti-EPX Antikörper (CF®740)

Verwendungszweck: Eosinophil Peroxidase(AHE-1), CF740 conjugate

Produktmerkmale: Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. AHE-1 recognizes human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. This MAb stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human eosinophils from a patient with hypereosinophilic syndrome

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: MCF-7 cells. Tonsil

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu EPX

Target: EPX (Eosinophil Peroxidase (EPX))

Andere Bezeichnung: Eosinophil Peroxidase

Hintergrund:

Synonyms: Eosinophil peroxidase heavy chain, EPER, EPO, EPP, EPX, EPX PEN, PERE

Gene Symbol: EPX

Tissue Expression: Eosinophils

Molekulargewicht: 70 kDa (55 kDa-Heavy Chain, 15 kDa-Light Chain)

Gen-ID: 8288

UniProt: P11678