Emerin Antikörper (AA 56-167) (CF®740)

Produktnummer ABIN7653448

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Emerin in WB, IHC, IF und IHC (fp). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Emerin Antikörper (AA 56-167) (CF®740) (ABIN7653448)

Target: Emerin (EMD)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Emerin Antikörper ist konjugiert mit CF®740

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: EMD-2167

Produktdetails anti-Emerin Antikörper (CF®740)

Bindungsspezifität: AA 56-167

Verwendungszweck: Emerin (Papillary Thyroid Carcinoma and EDMD Marker) (EMD/2167), CF740 conjugate

Produktmerkmale: Emerin is a member of the nuclear lamina associated protein family. It is ubiquitously expressed and localized to the nuclear membrane in normal cells. Mutations of the gene that encodes emerin result in the X-linked recessive disease Emery-Dreifuss muscular dystrophy (EDMD), which is characterized by slowly progressing contractures, skeletal muscle wasting and cardiomyopathy. Reportedly, lack of Emerin expression is one cause of EDMD. Emerin is involved in the association of the nuclear membrane with the lamina, and is localized specifically to desmosomes and fasciae adherents in the heart. Identification of nuclear membrane irregularities with anti-emerin antibody has been reported useful in diagnosing papillary thyroid carcinoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Emerin protein fragment (around aa 56-167)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: K-562, HeLa or Jurkat cells. Kidney or Skin

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Emerin

Target: Emerin (EMD)

Andere Bezeichnung: Emerin

Hintergrund:

Synonyms: EMD, Emerin, Emery Dreifuss muscular dystrophy (EDMD), STA

Gene Symbol: EMD

Molekulargewicht: 37 kDa

Gen-ID: 2010

UniProt: P50402