Desmoglein 3 Antikörper (AA 379-491) (CF®405S)

Produktnummer ABIN7653118

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Desmoglein 3 in . Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Desmoglein 3 Antikörper (AA 379-491) (CF®405S) (ABIN7653118)

Target: Desmoglein 3 (DSG3)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmoglein 3 Antikörper ist konjugiert mit CF®405S

Applikation: Bitte anfragen

Klon: DSG3-2796

Produktdetails anti-Desmoglein 3 Antikörper (CF®405S)

Bindungsspezifität: AA 379-491

Verwendungszweck: Desmoglein-3 (Squamous Cell Marker)(DSG3/2796), CF405S conjugate

Produktmerkmale: Recognizes a protein of 130 kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Research has shown that DSG3 has a very high sensitivity (80 % ) and specificity (100 %) in recognizing squamous cell carcinoma (SqCC). Therefore, DSG3 is considered a very important marker for lung SqCC and can be a useful ancillary marker to separate SqCC from other subtypes of lung cancer. Moreover, studies have shown that DSG3 expression in lung SqCC may indicate a poor prognosis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 379-491) human DSG3 protein

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: A431 cells. Skin or Lung Squamous Cell Carcinoma (SCC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Desmoglein 3

Target: Desmoglein 3 (DSG3)

Andere Bezeichnung: Desmoglein-3

Hintergrund:

Synonyms: 130 kDa pemphigus vulgaris antigen (PVA), Balding (Bal), Cadherin family member 6 (CDHF6), Desmoglein-3 (DSG3)

Gene Symbol: DSG3

Tissue Expression: Epidermal cells

Molekulargewicht: 130 kDa

Gen-ID: 1830

UniProt: P32926