Rekombinanter Desmocollin 2+3 Antikörper

Produktnummer ABIN7653102

Der Maus Monoklonal anti-Desmocollin 2+3 Antikörper (Klon rDSC2-3437) (ABIN7653102) detektiert spezifisch Desmocollin 2+3. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für Rekombinanter Desmocollin 2+3 Antikörper (ABIN7653102)

Target: Desmocollin 2+3

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Bitte anfragen

Klon: RDSC2-3437

Produktdetails

Verwendungszweck: Desmocollin-2/3(rDSC2/3437)

Produktmerkmale: Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein 1-3 and desmocollins. Desmosomal cadherins showed differentiation-specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Desmocollin-1 can be found in the upper layers. The expression of desmocollin-2 varies in the basal and suprabasal layers. And desmocollin-3 is expressed more evenly throughout the suprabasal layers. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human DSC2 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: HeLa cells. Skin tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Antigendetails

Target: Desmocollin 2+3

Andere Bezeichnung: Desmocollin-2/3

Hintergrund:

Synonyms: ARVD11, Cadherin family member 2, CDHF2, Desmocollin-2, Desmocollin-3, Desmosomal glycoprotein II and III, Desmosomal glycoprotein II, Desmosomal glycoprotein III, DG2, DGII/III, DSC2, DSC3

Gene Symbol: DSC2

Tissue Expression: Epidermal cells

Molekulargewicht: 110 kDa (DSC2), 100 kDa (DSC3)

Gen-ID: 1824

UniProt: Q02487