Desmin Antikörper (CF®740)

Produktnummer ABIN7653079

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Desmin in IHC und IHC (fp). Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus, Huhn und Hamster.

Kurzübersicht für Desmin Antikörper (CF®740) (ABIN7653079)

Target: Desmin (DES)

Reaktivität: Human, Ratte, Maus, Huhn, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmin Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: DES-1711

Produktdetails anti-Desmin Antikörper (CF®740)

Verwendungszweck: Desmin (Muscle Cell Marker) (DES/1711), CF740 conjugate

Produktmerkmale: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain alpha-helicalcoiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. The antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length desmin protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin) 1-2 μg/mL. Immunofluorescence 1-2 μg/mL Flow cytometry 0.5-1 μg/million cells. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: SJRH30 Cells. Uterus or Leiomyosarcoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Desmin

Target: Desmin (DES)

Andere Bezeichnung: Desmin

Hintergrund:

Synonyms: CMD1I, CSM1, CSM2, DES, Intermediate filament protein

Gene Symbol: DES

Tissue Expression: Muscle

Molekulargewicht: 52 kDa

Gen-ID: 1674

UniProt: P17661