Chp2 Antikörper (CF®647)

Produktnummer ABIN7652371

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Chp2 in WB und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Chp2 Antikörper (CF®647) (ABIN7652371)

Target: Chp2

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Chp2 Antikörper ist konjugiert mit CF®647

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: CPTC-CHP2-1

Produktdetails anti-Chp2 Antikörper (CF®647)

Verwendungszweck: Calcineurin B homologous protein 2 / HCC Antigen 520(CPTC-CHP2-1), CF647 conjugate

Produktmerkmale: Calcineurin B homologous protein 2, also known as hepatocellular carcinoma-associated antigen 520, is a 196 amino acid protein that contains 4 EF-hand domains and plays a potential role in transmembrane Na/H exchange. By binding to and activating NHE-1, CHP2 increases the pH and works to protect cells from serum deprivation-induced death. Though not typically detected in normal tissues, CHP2 is highly expressed in malignantly transformed cells and is therefore considered to be a tumor-associated antigen. Ectopic expression of CHP2 promotes proliferation of HEK293 cells and knockdown of CHP2 mRNA in HepG2 cells inhibits cell proliferation. Like calcineurin B, CHP2 can bind to and stimulate phosphatase activity of calcineurin A and activate the calcineurin/NFAT signaling pathway. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length CHP2 protein

Isotyp: IgG2c, kappa

Anwendungsinformationen

Applikationshinweise: Western blot 1-2 μg/mL, Immunofluorescence: 1-2 μg/mL, Optimal dilution for a specific application should be determined.

Kommentare:

Positive Control: MCF-7, PC-3, HT29, K562 or Jurkat cell lysates. Human appendix or testis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Chp2

Target: Chp2

Andere Bezeichnung: Calcineurin B homologous protein 2

Hintergrund:

Synonyms: Calcineurin B homologous protein 2, Calcineurin-like EF hand protein 2, HCA520, hepatocellular carcinoma antigen gene 520

Gene Symbol: CHP2

Molekulargewicht: 22 kDa

Gen-ID: 63928

UniProt: O43745