CD99 Antikörper (CF®740)

Produktnummer ABIN7652328

Dieses Anti-CD99-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD99 in IHC und IHC (fp). Geeignet für Human.

Kurzübersicht für CD99 Antikörper (CF®740) (ABIN7652328)

Target: CD99

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD99 Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: 12E7 MIC2-877

Produktdetails anti-CD99 Antikörper (CF®740)

Verwendungszweck: CD99 / MIC2 (Ewing's Sarcoma Marker)(12E7 + MIC2/877), CF740 conjugate

Produktmerkmale: This antibody recognizes a sialoglycoprotein of 27-32 kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32 kDa (p30/32).Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors.

Immunogen: Human acute lymphocytic leukemia T-cells (12E7), Recombinant human MIC2 protein (MIC2/877)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 5-10 uL/million cells. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistochemistry (formalin-fixed): 1:100-1:200 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Immunofluorescence 1:100-1:200. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: MOLT-4 cells. Pancreas or Ewing sarcoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu CD99

Target: CD99

Andere Bezeichnung: CD99

Hintergrund:

Synonyms: 12E7, E2 antigen, MIC 2X, MIC 2Y, MIC2, Protein MIC2, Surface antigen MIC2, T-cell surface glycoprotein E2

Gene Symbol: CD99

Tissue Expression: Lymphocytes|Ovary|Pancreatic islet cells|T-cells|Testis

Molekulargewicht: 27-32 kDa

Gen-ID: 4267

UniProt: P14209