Rekombinanter CD81 Antikörper (CF®488A)

Produktnummer ABIN7652082

Dieses Anti-CD81-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von CD81 in FACS, IHC, ExoS und IHC (fp). Geeignet für Human, Maus und Ratte.

Kurzübersicht für Rekombinanter CD81 Antikörper (CF®488A) (ABIN7652082)

Target: CD81

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CD81 Antikörper ist konjugiert mit CF®488A

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Exosome Staining (ExoS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: C81-2885R

Produktdetails anti-CD81 Antikörper (CF®488A)

Verwendungszweck: CD81 / TAPA-1 (C81/2885R), CF488A conjugate

Produktmerkmale: This antibody recognizes a protein of 26 kDa, identified as CD81 (Workshop VI, Code CD81.1). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspanin transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: B-Cell line derived from a Burkitt lymphoma

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 2-4 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Ramos, Daudi, Raji, U266, HUT-78, HUT-102, Jurkat, HL-60, KG1a, A293, A431, MG63, MCF-7, and human lymphocytes. Tonsil or liver tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu CD81

Target: CD81

Andere Bezeichnung: CD81

Hintergrund:

Synonyms: CD81, Target of the antiproliferative antibody 1 (TAPA-1), Tetraspanin-28, Tspan-28

Gene Symbol: CD81

Molekulargewicht: 26 kDa

Gen-ID: 975

UniProt: P60033

Pathways: Inositol Metabolic Process, Hepatitis C