Rekombinanter CD34 Antikörper (CF®740)

Produktnummer ABIN7651289

Der Kaninchen Monoklonal anti-CD34 Antikörper wird verwendet zum Nachweis von CD34 in Proben von Human und Ratte. Er wurde validiert für FACS, IHC und IHC (fp).

Kurzübersicht für Rekombinanter CD34 Antikörper (CF®740) (ABIN7651289)

Target: CD34

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CD34 Antikörper ist konjugiert mit CF®740

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: HPCA1-1806R

Produktdetails anti-CD34 Antikörper (CF®740)

Verwendungszweck: CD34 (Hematopoietic Stem Cell & Endothelial Marker) (HPCA1/1806R), CF740 conjugate

Produktmerkmale: This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85 % of angiosarcoma and Kaposi's sarcoma, but shows low specificity.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human HPCA1 protein

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Immunohistology (formalin) 2-4 μg/mL. Immunofluorescence 0.5-1 μg/mL Western blotting 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: KG-1 cells, Tonsil, or Angiosarcoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu CD34

Target: CD34

Andere Bezeichnung: CD34

Hintergrund:

Synonyms: Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

Gene Symbol: CD34

Tissue Expression: Endothelial cells|Hematopoietic stem cells

Molekulargewicht: 90-110 kDa

Gen-ID: 947

UniProt: P28906