CD163 Antikörper (AA 43-196) (Biotin)

Produktnummer ABIN7650853

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD163 in WB, IHC und IHC (fp). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD163 Antikörper (AA 43-196) (Biotin) (ABIN7650853)

Target: CD163

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD163 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: M130-1210

Produktdetails anti-CD163 Antikörper (Biotin)

Bindungsspezifität: AA 43-196

Verwendungszweck: CD163 (Monocyte & Macrophage Marker) (M130/1210), Biotin conjugate

Produktmerkmale: This MAb recognizes a protein of 140 kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression. Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human CD163 fragment (around aa 43-196)

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: HPBL cells. Skin, Spleen, Lung, Placenta or Histiocytoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu CD163

Target: CD163

Andere Bezeichnung: CD163

Hintergrund:

Synonyms: CD163, CD163 antigen, Macrophage-associated antigen, M130, CD163 Molecule, Hemoglobin scavenger receptor, MM130, Scavenger receptor cysteine rich type 1 protein M130

Gene Symbol: CD163

Tissue Expression: Monocytes/macrophages

Molekulargewicht: 140 kDa

Gen-ID: 9332