CD16 Antikörper (CF®740)

Produktnummer ABIN7650821

Der Maus Monoklonal Anti-CD16-Antikörper wurde für FACS und IF validiert. Er ist geeignet, CD16 in Proben von Human zu detektieren.

Kurzübersicht für CD16 Antikörper (CF®740) (ABIN7650821)

Target: CD16

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD16 Antikörper ist konjugiert mit CF®740

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: C16-1045

Produktdetails anti-CD16 Antikörper (CF®740)

Verwendungszweck: CD16(C16/1045), CF740 conjugate

Produktmerkmale: This antibody recognizes CD16 (Fci³RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. Two similar genes represent CD16, CD16A (Fci³RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fci³RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human CD16 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: K-562 or U-937 cells. Lymph nodes and tonsils.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu CD16

Target: CD16

Andere Bezeichnung: CD16

Hintergrund:

Synonyms: CD162, CLA, Cutaneous lymphocyte associated antigen, P-selectin glycoprotein ligand 1, PSGL1, Selectin P ligand, SELPLG

Gene Symbol: FCGR3A

Tissue Expression: Monocytes/macrophages|Neutrophils|NK cells

Molekulargewicht: 50-80 kDa

Gen-ID: 2214

UniProt: P08637

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process