S100A8/A9 Complex (Calprotectin) Antikörper (CF®740)

Produktnummer ABIN7650472

Dieses Maus Monoklonal-Antikörper erkennt spezifisch S100A8/A9 Complex (Calprotectin) in IHC, FACS, WB, IP und IHC (fp). Er zeigt eine Reaktivität gegenüber Human, Schwein, Rind (Kuh), Cat, Hund, Meerschweinchen, Affe, Kaninchen, Ratte, Maus, Pferd, Pavian und Ziege.

Kurzübersicht für S100A8/A9 Complex (Calprotectin) Antikörper (CF®740) (ABIN7650472)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Reaktivität: Human, Schwein, Rind (Kuh), Cat, Hund, Meerschweinchen, Affe, Kaninchen, Ratte, Maus, Pferd, Pavian, Ziege

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser S100A8/A9 Complex (Calprotectin) Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: MAC387

Produktdetails anti-S100A8/A9 Complex (Calprotectin) Antikörper (CF®740)

Verwendungszweck: Calgranulin B / MRP-8/-14 / MAC 387 / S100A8/A9 / Macrophage Ag(MAC387), CF740 conjugate

Produktmerkmale: Calprotectin is a heteroduplex of Protein S100-A8/A9 (also called MRP-8/14, or Calgranulin A/B). This antibody recognizes the 14 kDa B component (Protein S100-A9) of the complex. Protein S100-A9 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. Altered expression of this protein is associated with the disease cystic fibrosis. This antibody reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Affinity purified monocyte membrane preparation

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Tonsil, lymph node, or spleen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu S100A8/A9 Complex (Calprotectin)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Andere Bezeichnung: Calprotectin

Hintergrund:

Synonyms: Calprotectin, Protein S100-A9, Myeloid-related protein 14, MRP-14, Calgranulin B, CAGB, Calprotectin L1H Subunit

Tissue Expression: Granulocytes|Monocytes/macrophages

Molekulargewicht: 12-14 kDa (doublet)

Gen-ID: 6279

Pathways: S100 Proteine