Rekombinanter Biotin Antikörper (CF®594)

Produktnummer ABIN7650071

Der Kaninchen Monoklonal anti-Biotin Antikörper wird verwendet zum Nachweis von Biotin in Proben von Diverse Spezies. Er wurde validiert für IHC und IHC (fp).

Kurzübersicht für Rekombinanter Biotin Antikörper (CF®594) (ABIN7650071)

Target: Biotin

Antikörpertyp: Recombinant Antibody

Reaktivität: Diverse Spezies

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Biotin Antikörper ist konjugiert mit CF®594

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: BTN-2032R

Produktdetails anti-Biotin Antikörper (CF®594)

Verwendungszweck: Biotin (Vitamin B7 or Vitamin H) (BTN/2032R), CF594 conjugate

Produktmerkmale: This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Biotinylated sheep immunoglobulin

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. In situ Nucleic Acid Hybridization. No special pretreatment is required for the immunohistochemical staining of formalin-fixed paraffin-embedded tissues.. Western blotting. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Biotinylated proteins in solution or on tissues

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Biotin

Target: Biotin

Substanzklasse: Chemical

Hintergrund:

Synonyms: Vitamin B7 or Vitamin H

Gene Symbol: Not Applicable

Molekulargewicht: 244Da