CD147 Antikörper (CF®740)

Produktnummer ABIN7650001

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD147 in IHC und IHC (fp). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD147 Antikörper (CF®740) (ABIN7650001)

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD147 Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: BSG-963

Produktdetails anti-CD147 Antikörper (CF®740)

Verwendungszweck: CD147(BSG/963), CF740 conjugate

Produktmerkmale: This MAb recognizes extracellular epitope of human CD147. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human BSG protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 1:100-1:200. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HSB2 cells. Renal Cell, Colon or Testicular carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Andere Bezeichnung: CD147

Hintergrund:

Synonyms: 5A11 Antigen, 5F7, Basigin, Blood brain barrier HT7 antigen BSG, Extracellular Matrix Metalloproteinase Inducer (EMMPRIN), Leukocyte Activation Antigen M6, Neurothelin, OK Blood Group Antigen, TCSF, Tumor Cell-derived Collagenase Stimulatory Factor

Gene Symbol: BSG

Molekulargewicht: 35 kDa (non-reduced), 40 kDa (reduced)

Gen-ID: 682

UniProt: P35613

Pathways: S100 Proteine