ASRGL1 Antikörper (CF®740)
Kurzübersicht für ASRGL1 Antikörper (CF®740) (ABIN7649685)
Target
Alle ASRGL1 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Verwendungszweck
- ASRGL1 (Asparaginase Like 1) (CRASH/1289), CF740 conjugate
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Produktmerkmale
- ASRGL1 (Asparaginase-like protein 1), also known as CRASH, is a 308 amino acid protein belonging to the Ntn-hydrolase family. Asparaginases utilize asparagine as a substrate to produce aspartic acid and ammonia. ASRGL1 has been identified as a autoantigenic protein that is present in the mid-piece of sperm after obstruction of the male reproductive tract. ASRGL1 is expressed highly in testis, but is also expressed in brain, kidney and gastrointestinal tissues. High levels of ASRGL1 have also been identified in ovarian, uterine and mammary tumors in comparison with normal tissues of the same origin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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Immunogen
- Recombinant human full-length ASRGL1
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Isotyp
- IgG1
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Applikationshinweise
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user
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Kommentare
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Positive Control: HeLa cells. Fallopian Tube, Endometrium, Uterus or Cervix.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 0.1 mg/mL
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Buffer
- PBS, 0.1 % rBSA, 0.05 % azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Protect from light
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Lagerung
- 4 °C
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Informationen zur Lagerung
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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Haltbarkeit
- 24 months
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- ASRGL1 (Asparaginase Like 1 (ASRGL1))
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Andere Bezeichnung
- ASRGL1
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Hintergrund
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Synonyms: ASRGL1, isoaspartyl peptidase, L-asparaginase, ALP1, beta-aspartyl-peptidase, CRASH, L asparagine amidohydrolase
Gene Symbol: ASRGL1
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Molekulargewicht
- 17-25 kDa
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Gen-ID
- 80150
Target
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