ADIPOQ Antikörper (CF®740)

Produktnummer ABIN7649181

Dieses Anti-ADIPOQ-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ADIPOQ in WB, IHC und IHC (fp). Geeignet für Human.

Kurzübersicht für ADIPOQ Antikörper (CF®740) (ABIN7649181)

Target: ADIPOQ (Adiponectin (ADIPOQ))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ADIPOQ Antikörper ist konjugiert mit CF®740

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: ADPN-1370

Produktdetails anti-ADIPOQ Antikörper (CF®740)

Verwendungszweck: Adiponectin (Marker of Obesity) (ADPN/1370), CF740 conjugate

Produktmerkmale: Adiponectin is an adipocytokine, hormones produced in adipose tissue. Adiponectin is abundantly present in plasma and has insulin like effect on glucose levels in the blood. Plasma adiponectin levels are low in insulin resistant patients who are obese, have diabetes mellitus type 2 or HIV-lipodystrophy. In women adiponectin levels tend to be higher than in men, which may be due to androgens suppressing adiponectin levels. Furthermore adiponectin and leptin are both indicated in regulating body weight through direct action on the hypothalamus, influencing appetite. Obese people have low adiponectin levels while levels in anorexia patients are high. Adiponectin acts as ligand for various receptors, two of which have been identified, one probably involved in carbohydrate assimilation, the other in tuning the rate of metabolism. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Adiponectin protein fragment

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology (formalin) 1-2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. ELISA For coating, order Ab without BSA. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HeLa cells or Kidney

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu ADIPOQ

Target: ADIPOQ (Adiponectin (ADIPOQ))

Andere Bezeichnung: Adiponectin

Hintergrund:

Synonyms: ADPN, ACRP30, Adipocyte Complement-Related Protein, Gelatin binding protein

Gene Symbol: ADIPOQ

Molekulargewicht: 26-30 kDa

Gen-ID: 9370