PUMA Antikörper (AA 131-180)

Produktnummer ABIN731528

Der Kaninchen Polyklonal Anti-PUMA-Antikörper wurde für WB, ELISA, IP, FACS, IHC (p), IF (cc), IF (p) und IHC (fro) validiert. Er ist geeignet, PUMA in Proben von Human, Ratte und Schwein zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für PUMA Antikörper (AA 131-180) (ABIN731528)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reaktivität: Human, Ratte, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PUMA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-PUMA Antikörper

Bindungsspezifität: AA 131-180

Kreuzreaktivität: Human, Schwein, Ratte

Homologie: Mouse,Dog,Cow

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PUMA

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
IP(1-2 μg)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-PUMA Antikörper (ABIN731528)

Su, Shi, Yan, Xi, Su: "Anticancer bioactive peptides suppress human colorectal tumor cell growth and induce apoptosis via modulating the PARP-p53-Mcl-1 signaling pathway." in: Acta pharmacologica Sinica, Vol. 36, Issue 12, pp. 1514-9, (2016) (PubMed).

Qiu, Cai, Gao, Gu, Liu: "A small peptide derived from p53 linker region can resume the apoptotic activity of p53 by sequestering iASPP with p53." in: Cancer letters, Vol. 356, Issue 2 Pt B, pp. 910-7, (2014) (PubMed).

Zhang, Liu, Xia, Zhang, Zhang, Zhao, Chao, Jiang, Jiang: "Epithelial-mesenchymal transition is necessary for acquired resistance to cisplatin and increases the metastatic potential of nasopharyngeal carcinoma cells." in: International journal of molecular medicine, Vol. 33, Issue 1, pp. 151-9, (2013) (PubMed).

Details zu PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Andere Bezeichnung: PUMA

Hintergrund:

Synonyms: JFY1, PUMA, JFY-1, Bcl-2-binding component 3, p53 up-regulated modulator of apoptosis, BBC3

Background: Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Isoform 3 fails to show any growth-inhibitory or apoptotic activity.

Gen-ID: 27113

UniProt: Q96PG8

Pathways: p53 Signalweg, Positive Regulation of Endopeptidase Activity