F-Actin Antikörper (AA 1-50)

Produktnummer ABIN731498

Der Kaninchen Polyklonal Anti-F-Actin-Antikörper wurde für FACS, WB, ELISA, IHC (p), IF (cc) und IF (p) validiert. Er ist geeignet, F-Actin in Proben von Human, Maus, Ratte, Huhn und Hund zu detektieren. Es sind 14+ Publikationen verfügbar.

Kurzübersicht für F-Actin Antikörper (AA 1-50) (ABIN731498)

Target: F-Actin

Reaktivität: Human, Maus, Ratte, Huhn, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser F-Actin Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-F-Actin Antikörper

Bindungsspezifität: AA 1-50

Kreuzreaktivität: Huhn, Hund, Human, Maus, Ratte

Homologie: Cow,Sheep,Pig

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human F-actin capping protein alpha subunit

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-F-Actin Antikörper (ABIN731498)

Zhang, Zhao, Yu, Liu, Hao, Zhang, Ge, Min, Shen, Li, Kou, Ma, Li: "Proteome analysis of egg yolk after exposure to zinc oxide nanoparticles." in: Theriogenology, Vol. 95, pp. 154-162, (2018) (PubMed).

Clark, Garcia Galindo, Patel, Parry, Stoll, Yavorski, Pinkason, Johnson, Walker, Johnson, Sexton, Coppola, Blanck: "The human, F-actin-based cytoskeleton as a mutagen sensor." in: Cancer cell international, Vol. 17, pp. 121, (2018) (PubMed).

Guo, Liu: "Nogo receptor knockdown and ciliary neurotrophic factor attenuate diabetic retinopathy in streptozotocin-induced diabetic rats." in: Molecular medicine reports, Vol. 16, Issue 2, pp. 2030-2036, (2017) (PubMed).

Siar, Rahman, Tsujigiwa, Mohamed Om Alblazi, Nagatsuka, Ng: "Invadopodia proteins, cortactin, N-WASP and WIP differentially promote local invasiveness in ameloblastoma." in: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, Vol. 45, Issue 8, pp. 591-8, (2016) (PubMed).

Chen, Chen, Lu, Liu, Jiang, Ma: "Cofilin is correlated with sperm quality and influences sperm fertilizing capacity in humans." in: Andrology, Vol. 4, Issue 6, pp. 1064-1072, (2016) (PubMed).

Li, Zhang, Chen, Li, Zhang: "Human eccrine sweat gland cells reconstitute polarized spheroids when subcutaneously implanted with Matrigel in nude mice." in: Journal of molecular histology, Vol. 47, Issue 5, pp. 485-90, (2016) (PubMed).

Murakami, Nakanishi, Hirotani, Ohni, Tang, Masuda, Enomoto, Sakurai, Amano, Yamada, Nemoto: "Roles of Ras Homolog A in Invasive Ductal Breast Carcinoma." in: Acta histochemica et cytochemica, Vol. 49, Issue 5, pp. 131-140, (2016) (PubMed).

Yuan, Lv, Li, Gao, Song, Zhang, Xing, Kong, Wang, Li, Zhou, Gao, Xiao, Yin, Wei, He, Zhang: "Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis." in: Nature cell biology, Vol. 17, Issue 9, pp. 1169-81, (2015) (PubMed).

Du, Jiang, Xu, An, Liu, Li, Zhang: "Damage of hippocampal neurons in rats with chronic alcoholism." in: Neural regeneration research, Vol. 9, Issue 17, pp. 1610-5, (2014) (PubMed).

Zhong, Zhang, Wang, Qin: "Regulation of fibrochondrogenesis of mesenchymal stem cells in an integrated microfluidic platform embedded with biomimetic nanofibrous scaffolds." in: PLoS ONE, Vol. 8, Issue 4, pp. e61283, (2013) (PubMed).

Kang, Liu, Fan, Deng: "A potential gravity-sensing role of vascular smooth muscle cell glycocalyx in altered gravitational stimulation." in: Astrobiology, Vol. 13, Issue 7, pp. 626-36, (2013) (PubMed).

Su, Peng, Jiang, Wu, Qiao, Li, Qi: "In vitro and in vivo evaluations of nano-hydroxyapatite/polyamide 66/glass fibre (n-HA/PA66/GF) as a novel bioactive bone screw." in: PLoS ONE, Vol. 8, Issue 7, pp. e68342, (2013) (PubMed).

Zhao, Wang, Kong, Zhang, Meng, Jiang, Wu, Yu, Jin: "Hepatoma cell line HepG2.2.15 demonstrates distinct biological features compared with parental HepG2." in: World journal of gastroenterology : WJG, Vol. 17, Issue 9, pp. 1152-9, (2011) (PubMed).

Details zu F-Actin

Target: F-Actin

Hintergrund:

Synonyms: CapZ alpha-1, F-actin-capping protein subunit alpha-1, CAZA1_HUMAN

Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. May play a role in the formation of epithelial cell junctions.

Gen-ID: 60

UniProt: P52907