AKT 1/2/3 Antikörper (pSer473)

Produktnummer ABIN729298

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch AKT 1/2/3 in WB, ELISA, IF (cc), IF (p), IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Kaninchen, Huhn und Fisch und wurde in 6+ Publikationen erwähnt.

Kurzübersicht für AKT 1/2/3 Antikörper (pSer473) (ABIN729298)

Target: AKT 1/2/3

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen, Huhn, Fisch

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AKT 1/2/3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-AKT 1/2/3 Antikörper

Bindungsspezifität: pSer473

Kreuzreaktivität: Huhn, Rind (Kuh), Fisch, Human, Maus, Kaninchen, Ratte

Homologie: Dog,Cow,Sheep

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Ser473

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-AKT 1/2/3 Antikörper (ABIN729298)

Li, Jia, Ji, Gong, Wang, Zhang, Wang, Zang: "The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats." in: International journal of molecular medicine, Vol. 38, Issue 3, pp. 767-75, (2016) (PubMed).

Xu, Xu, Luan, Jiang, Tian, Zhang: "Cardioprotection against experimental myocardial ischemic injury using cornin." in: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, Vol. 49, Issue 2, pp. e5039, (2016) (PubMed).

Santos, Mesquita, Barros-Silva, Jerónimo, Henrique, Morais, Paulo, Teixeira: "Uncovering potential downstream targets of oncogenic GRPR overexpression in prostate carcinomas harboring ETS rearrangements." in: Oncoscience, Vol. 2, Issue 5, pp. 497-507, (2015) (PubMed).

Liang, Zeng, Wang, Shen, Li, Ma, Ci, Yu, Jia, Sun, Liu, Liu, Li, Yu, Chen, Jia: "Histone demethylase RBP2 induced by Helicobactor Pylori CagA participates in the malignant transformation of gastric epithelial cells." in: Oncotarget, Vol. 5, Issue 14, pp. 5798-807, (2015) (PubMed).

Tang, Liu, Dong, Li, Li, Lin, Zheng, Hou, Li: "The cardioprotective effects of citric Acid and L-malic Acid on myocardial ischemia/reperfusion injury." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2013, pp. 820695, (2013) (PubMed).

Details zu AKT 1/2/3

Target: AKT 1/2/3

Andere Bezeichnung: AKT1/2/3

Hintergrund:

Synonyms: Akt, RAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, RAC-PK-alpha, Akt1

Background: AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility, prohibitin (PHB), playing an important role in cell metabolism and proliferation, and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

Gen-ID: 24185

UniProt: P47196