GSTA3 Antikörper (N-Term)

Produktnummer ABIN7603173

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GSTA3 in WB und FACS. Er zeigt eine Reaktivität gegenüber Maus und Ratte.

Kurzübersicht für GSTA3 Antikörper (N-Term) (ABIN7603173)

Target: GSTA3 (Glutathione S-Transferase alpha 3 (GSTA3))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GSTA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-GSTA3 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Anti-Gsta3 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Gsta3 Antibody Picoband® (ABIN7603173). Tested in Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse Gsta3, which shares 57.9% and 89.5% amino acid (aa) sequence identity with human and rat Gsta3, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
1. Board, P. G. Identification of cDNAs encoding two human alpha class glutathione transferases (GSTA3 and GSTA4) and the heterologous expression of GSTA4-4. Biochem. J. 330: 827-831, 1998. 2. Morel, F., Rauch, C., Coles, B., Le Ferrec, E., Guillouzo, A. The human glutathione transferase alpha locus: genomic organization of the gene cluster and functional characterization of the genetic polymorphism in the hGSTA1 promoter. Pharmacogenetics 12: 277-286, 2002. 3. Suzuki, T., Johnston, P. N., Board, P. G. Structure and organization of the human alpha class glutathione S-transferase genes and related pseudogenes. Genomics 18: 680-686, 1993.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu GSTA3

Target: GSTA3 (Glutathione S-Transferase alpha 3 (GSTA3))

Andere Bezeichnung: Gsta3

Hintergrund:

Synonyms: LIM/homeobox protein Lhx1, LIM homeobox protein 1, Homeobox protein Lim-1, hLim-1, LHX1, LIM-1, LIM1

Tissue Specificity: Expressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58 % of acute myeloid leukemia (AML) cell lines.

Background: Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class genes that are located in a cluster mapped to chromosome 6. Genes of the alpha class are highly related and encode enzymes with glutathione peroxidase activity. However, during evolution, this alpha class gene diverged accumulating mutations in the active site that resulted in differences in substrate specificity and catalytic activity. The enzyme encoded by this gene catalyzes the double bond isomerization of precursors for progesterone and testosterone during the biosynthesis of steroid hormones. An additional transcript variant has been identified, but its full length sequence has not been determined.

Molekulargewicht: 28 kDa

Gen-ID: 2940

UniProt: P30115