GAP43 Antikörper (N-Term)

Produktnummer ABIN7603111

Der Kaninchen Polyklonal Anti-GAP43-Antikörper wurde für WB, IHC, IF und FACS validiert. Er ist geeignet, GAP43 in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für GAP43 Antikörper (N-Term) (ABIN7603111)

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GAP43 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-GAP43 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Anti-GAP43 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-GAP43 Antibody Picoband® (ABIN7603111). Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human GAP43, which shares 95.2% amino acid (aa) sequence identity with both mouse and rat GAP43.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Rat
Immunofluorescence, 5 μg/mL, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Kosik, K. S., Orecchio, L. D., Bruns, G. A. P., Benowitz, L. I., MacDonald, G. P., Cox, D. R., Neve, R. L. Human GAP-43: its deduced amino acid sequence and chromosomal localization in mouse and human. Neuron 1: 127-132, 1988. 2. Reeves, R. H., O'Hara, B. F., Gearhart, J. D. Localization on mouse chromosome 16 of Smst and Gap43: a conserved synteny with human chromosome 3. (Abstract) Cytogenet. Cell Genet. 51: 1064, 1989. 3. Strittmatter, S. M., Fankhauser, C., Huang, P. L., Mashimo, H., Fishman, M. C. Neuronal pathfinding is abnormal in mice lacking the neuronal growth cone protein GAP-43. Cell 80: 445-452, 1995.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu GAP43

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Andere Bezeichnung: GAP43

Hintergrund:

Synonyms: Neuromodulin, Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, pp46, GAP43

Tissue Specificity: Isoform 1 and isoform 2 are expressed in liver, colon and small intestine. Isoform 2 but not isoform 1 is expressed in kidney. Isoform 1 and isoform 2 are not expressed in esophagus. Not expressed in skin.

Background: Growth Associated Protein 43 (GAP43) is a protein encoded by the GAP43 gene in humans. It is mapped to 3q13.31. The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Molekulargewicht: 38-43 kDa

Gen-ID: 2596

UniProt: P17677