EHD2 Antikörper (Middle Region)

Produktnummer ABIN7603043

Produktdetails anti-EHD2 Antikörper

Target: EHD2 (EH-Domain Containing 2 (EHD2))

Bindungsspezifität: Middle Region

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EHD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Verwendungszweck: Anti-EHD2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-EHD2 Antibody Picoband® (ABIN7603043). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human EHD2, which shares 100% and 95% amino acid (aa) sequence identity with mouse and rat EHD2, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. Daumke, O., Lundmark, R., Vallis, Y., Martens, S., Butler, P. J. G., McMahon, H. T. Architectural and mechanistic insights into an EHD ATPase involved in membrane remodelling. Nature 449: 923-927, 2007. 2. Guilherme, A., Soriano, N. A., Bose, S., Holik, J., Bose, A., Pomerleau, D. P., Furcinitti, P., Leszyk, J., Corvera, S., Czech, M. P. EHD2 and the novel EH domain binding protein EHBP1 couple endocytosis to the actin cytoskeleton. J. Biol. Chem. 279: 10593-10605, 2004. 3. Pohl, U., Smith, J. S., Tachibana, I., Ueki, K., Lee, H. K., Ramaswamy, S., Wu, Q., Mohrenweiser, H. W., Jenkins, R. B., Louis, D. N. EHD2, EHD3, and EHD4 encode novel members of a highly conserved family of EH domain-containing proteins. Genomics 63: 255-262, 2000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu EHD2

Target: EHD2 (EH-Domain Containing 2 (EHD2))

Andere Bezeichnung: EHD2 (EHD2 Produkte)

Hintergrund:

Synonyms: Calretinin, CR, 29 kDa calbindin, CALB2, CAB29

Tissue Specificity: Brain.

Background: EH-domain containing 2, also known as EHD2, is a human gene[5] belonging to the EHD protein family. This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.

Molekulargewicht: 61 kDa

Gen-ID: 30846

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development