Leucine Rich Transmembrane and 0-Methyltransferase Domain Containing (LRTOMT) (Middle Region) Antikörper

Produktnummer ABIN7602986

Dieses Anti--Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von in WB und IHC. Geeignet für Human, Maus und Ratte.

Kurzübersicht für Leucine Rich Transmembrane and 0-Methyltransferase Domain Containing (LRTOMT) (Middle Region) Antikörper (ABIN7602986)

Target: Leucine Rich Transmembrane and 0-Methyltransferase Domain Containing (LRTOMT)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails

Bindungsspezifität: Middle Region

Verwendungszweck: Anti-LRTOMT Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-LRTOMT Antibody (ABIN7602986). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human LRTOMT.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/mL, Human, Mouse, Rat
1. Charif M, Bounaceur S, Abidi O, Nahili H, Rouba H, Kandil M, Boulouiz R, Barakat A (December 2012). "The c.242G>A mutation in LRTOMT gene is responsible for a high prevalence of deafness in the Moroccan population". Molecular Biology Reports. 39 (12): 11011-6. 2. "Entrez Gene: Leucine rich transmembrane and O-methyltransferase domain containing" 3. Riahi Z, Bonnet C, Zainine R, Louha M, Bouyacoub Y, Laroussi N, Chargui M, Kefi R, Jonard L, Dorboz I, Hardelin JP, Salah SB, Levilliers J, Weil D, McElreavey K, Boespflug OT, Besbes G, Abdelhak S, Petit C (2014). "Whole exome sequencing identifies new causative mutations in Tunisian families with non-syndromic deafness". PLoS One. 9 (6): e99797.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Antigendetails

Target: Leucine Rich Transmembrane and 0-Methyltransferase Domain Containing (LRTOMT)

Andere Bezeichnung: LRTOMT

Hintergrund:

Synonyms: Transmembrane O-methyltransferase, Catechol O-methyltransferase 2, Protein LRTOMT2, LRTOMT, COMT2

Background: Leucine rich transmembrane and O-methyltransferase domain containing is a protein that in humans is encoded by the LRTOMT gene. It is mapped to 11q13.4. This gene has evolved in primates as a fusion of two ancestral neighboring genes, Lrrc51 and Tomt, which exist as two independent genes in rodents. The fusion gene contains some shared exons, but encodes structurally unrelated proteins, LRTOMT1 and LRTOMT2. Those variants that utilize the more centromere-proximal 3' terminal exon (short transcript form) encode LRTOMT1, while those variants that use a more centromere-distal 3' terminal exon (long transcript form) encode the LRTOMT2 protein. There is a small region within one of the exons of this gene that contains overlapping alternate reading frames for both LRTOMT1 and LRTOMT2. LRTOMT1 shares similarity with the protein encoded by mouse Lrrc51, while LRTOMT2 shares similarity with the protein encoded by mouse Tomt. Alternative splicing results in multiple transcript variants, encoding different isoforms of both LRTOMT1 and LRTOMT2. The LRTOMT1 protein is a leucine-rich repeat-containing protein, while the LRTOMT2 protein is a catechol-O-methyltransferase that catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to a hydroxyl group of catechols and is essential for auditory and vestibular function. Mutations in this gene have been associated with nonsyndromic deafness.

Molekulargewicht: 28 kDa

Gen-ID: 220074

Pathways: Sensory Perception of Sound