T-Bet Antikörper (Middle Region)

Produktnummer ABIN7602980

Dieses Anti-T-Bet-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von T-Bet in WB und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für T-Bet Antikörper (Middle Region) (ABIN7602980)

Target: T-Bet

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser T-Bet Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-T-Bet Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: Anti-T-bet/Tbx21 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-T-bet/Tbx21 Antibody (ABIN7602980). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human T-bet/Tbx21, identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat
1. Akahoshi, M., Obara, K., Hirota, T., Matsuda, A., Hasegawa, K., Takahashi, N., Shimizu, M., Nakashima, K., Cheng, L., Doi, S., Fujiwara, H., Miyatake, A., and 10 others. Functional promoter polymorphism in the TBX21 gene associated with aspirin-induced asthma. Hum. Genet. 117: 16-26, 2005. 2. Woo, C. H., Shin, S. G., Koh, S. H., Lim, J. H. TBX21 participates in innate immune response by regulating Toll-like receptor 2 expression in Streptococcus pneumoniae infections. Molec. Oral Microbiol. 29: 233-243, 2014. 3. Yi, W., Zhang, P., Liang, Y., Zhou, Y., Shen, H., Fan, C., Moorman, J. P., Yao, Z. Q., Jia, Z., Zhang, Y. T-bet-mediated Tim-3 expression dampens monocyte function during chronic hepatitis C virus infection. Immunology 150: 301-311, 2016.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu T-Bet

Target: T-Bet

Andere Bezeichnung: TBX21

Hintergrund:

Synonyms: T-box transcription factor TBX21, T-box protein 21, T-cell-specific T-box transcription factor T-bet, Transcription factor TBLYM, TBX21, TBET, TBLYM

Tissue Specificity: Expressed in the brain, with expression in the hippocampus, the neuropil in the dentate gyrus, where expression is higher in the outer half of the molecular layer than in the inner half, and in the neuropil of CA4 and CA3.

Background: T-box transcription factor TBX21 is a protein that in humans is encoded by the TBX21 gene. It is mapped to 17q21.32. This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells.

Molekulargewicht: 65 kDa

Gen-ID: 30009