BCAM Antikörper (Middle Region)

Produktnummer ABIN7602976

Der Kaninchen Polyklonal Anti-BCAM-Antikörper wurde für WB, IHC, FACS, ICC und IF validiert. Er ist geeignet, BCAM in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für BCAM Antikörper (Middle Region) (ABIN7602976)

Target: BCAM (Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BCAM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-BCAM Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: Anti-CD239/BCAM Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-/BCAM Antibody (ABIN7602976). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human CD239/BCAM, which shares 84.4% amino acid (aa) sequence identity with both mouse and rat CD239/BCAM.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Campbell, I. G., Foulkes, W. D., Senger, G., Trowsdale, J., Garin-Chesa, P., Rettig, W. J. Molecular cloning of the B-CAM cell surface glycoprotein of epithelial cancers: a novel member of the immunoglobulin superfamily. Cancer Res. 54: 5761-5765, 1994. 2. Cook, P. J. L. The Lutheran-secretor recombination fraction in man: a possible sex difference. Ann. Hum. Genet. 28: 393-401, 1965. 3. Daniels, G. L., Le Pennec, P. Y., Rouger, P., Salmon, C., Tippett, P. The red cell antigens Au(a) and Au(b) belong to the Lutheran system. Vox Sang. 60: 191-192, 1991.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu BCAM

Target: BCAM (Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM))

Andere Bezeichnung: BCAM

Hintergrund:

Synonyms: Basal cell adhesion molecule, Auberger B antigen, B-CAM cell surface glycoprotein, F8/G253 antigen, Lutheran antigen, Lutheran blood group glycoprotein, CD239, BCAM, LU, MSK19

Tissue Specificity: Wide tissue distribution (highest in the pancreas and very low in brain). Closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls.

Background: Basal cell adhesion molecule, also known as Lutheran antigen, is a plasma membraneglycoprotein that in humans is encoded by the BCAM gene. It is mapped to 19q13.32. This gene encodes Lutheran blood group glycoprotein, a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five extracellular immunoglobulin domains, a single transmembrane domain, and a short C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu (a-b-), of the Lutheran blood group. Two transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 70 kDa

Gen-ID: 4059

UniProt: P50895