TFF1 Antikörper (C-Term)

Produktnummer ABIN7602931

Der Kaninchen Polyklonal Anti-TFF1-Antikörper wurde für WB, IF und ICC validiert. Er ist geeignet, TFF1 in Proben von Human zu detektieren.

Kurzübersicht für TFF1 Antikörper (C-Term) (ABIN7602931)

Target: TFF1 (Trefoil Factor 1 (TFF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TFF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-TFF1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-TFF1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-TFF1 Antibody Picoband® (ABIN7602931). Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TFF1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. Chutipongtanate, S., Nakagawa, Y., Sritippayawan, S., Pittayamateekul, J., Parichatikanond, P., Westley, B. R., May, F. E. B., Malasit, P., Thongboonkerd, V.Identification of human urinary trefoil factor 1 as a novel calcium oxalate crystal growth inhibitor.J. Clin. Invest. 115: 3613-3622, 2005. 2. Hanby, A. M., Poulsom, R., Singh, S., Elia, G., Jeffery, R. E., Wright, N. A.Spasmolytic polypeptide is a major antral peptide: distribution of the trefoil peptides human spasmolytic polypeptide and pS2 in the stomach.Gastroenterology 105: 1110-1116, 1993. 3. Roberts, M., Wallace, J., Jeltsch, J.-M., Berry, M.The 5-prime flanking region of the human pS2 gene mediates its transcriptional activation by estrogen in MCF-7 cells.Biochem. Biophys. Res. Commun. 151: 306-313, 1988.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu TFF1

Target: TFF1 (Trefoil Factor 1 (TFF1))

Andere Bezeichnung: TFF1

Hintergrund:

Synonyms: Trefoil factor 1,Protein pS2,Tff1,Bcei, Ps2,

Tissue Specificity: Strong expression found in normal gastric mucosa. Not detected in the small or large intestine nor in normal mammary gland.

Background: TFF1 (Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to that of nephrocalcin, and inhibition was dose dependent and inhibited by TFF1 antisera, particularly by antisera ed to the TFF1 C terminus. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.

Molekulargewicht: 12 kDa

Gen-ID: 7031

UniProt: P04155

Pathways: EGFR Signaling Pathway