POGLUT1 Antikörper (C-Term)

Produktnummer ABIN7602910

Der Kaninchen Polyklonal Anti-POGLUT1-Antikörper wurde für WB, IHC, IF und ICC validiert. Er ist geeignet, POGLUT1 in Proben von Human und Maus zu detektieren.

Kurzübersicht für POGLUT1 Antikörper (C-Term) (ABIN7602910)

Target: POGLUT1 (Protein O-Glucosyltransferase 1 (POGLUT1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POGLUT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-POGLUT1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-POGLUT1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-POGLUT1 Antibody Picoband® (ABIN7602910). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at C-terminus of human POGLUT1, which shares 92.6% and 88.9% amino acid (aa) sequence identity with mouse and rat POGLUT1, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. Basmanav, F. B., Oprisoreanu, A.-M., Pasternack, S. M., Thiele, H., Fritz, G., Wenzel, J., Grosser, L., Wehner, M., Wolf, S., Fagerberg, C., Bygum, A., Altmuller, J., and 9 others. Mutations in POGLUT1, encoding protein O-glucosyltransferase 1, cause autosomal-dominant Dowling-Degos disease. Am. J. Hum. Genet. 94: 135-143, 2014. 2. Chu, Q., Liu, L., Wang, W. Overexpression of hCLP46 enhances Notch activation and regulates cell proliferation in a cell type-dependent manner. Cell Prolif. 46: 254-262, 2013. 3. Hanneken, S., Rutten, A., Eigelshoven, S., Braun-Falco, M., Pasternack, S. M., Ruzicka, T., Nothen, M. M., Betz, R. C., Kruse, R. Morbus Galli-Galli: klinische und histopathologische Untersuchung anhand einer Fallserie von 18 Patienten. Hautarzt 62: 842-851, 2011.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu POGLUT1

Target: POGLUT1 (Protein O-Glucosyltransferase 1 (POGLUT1))

Andere Bezeichnung: POGLUT1

Hintergrund:

Synonyms: Intraflagellar transport protein 88 homolog, Recessive polycystic kidney disease protein Tg737 homolog, Tetratricopeptide repeat protein 10, TPR repeat protein 10, IFT88, TG737, TTC10

Tissue Specificity: Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.

Background: This gene encodes a protein with both O-glucosyltransferase and O-xylosyltransferase activity which localizes to the lumen of the endoplasmic reticulum. This protein has a carboxy-terminal KTEL motif which is predicted to function as an endoplasmic reticulum retention signal. This gene is an essential regulator of Notch signalling and likely plays a role in cell fate and tissue formation during development. It may also play a role in the pathogenesis of leukemia. Mutations in this gene have been associated with the autosomal dominant genodermatosis Dowling-Degos disease 4. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 55 kDa

Gen-ID: 56983

Pathways: Notch Signalweg