HSF1 Antikörper (C-Term)

Produktnummer ABIN7602894

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch HSF1 in WB, IHC und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für HSF1 Antikörper (C-Term) (ABIN7602894)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-HSF1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-HSF1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-HSF1 Antibody Picoband® (ABIN7602894). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HSF1, identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL/mL, Human
Flow Cytometry (Fixed), 1-3 μg/mL/1x10^6 cells, Human
1. Christians, E., Davis, A. A., Thomas, S. D., Benjamin, I. J. Maternal effect of Hsf1 on reproductive success. Nature 407: 693-694, 2000. 2. Schuetz, T. J. Personal Communication. Boston, Mass. 10/3/1991.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Andere Bezeichnung: HSF1

Hintergrund:

Synonyms: C-reactive protein, C-reactive protein (1-205), CRP, PTX1

Tissue Specificity: Found in plasma.

Background: HSF1(HEAT-SHOCK TRANSCRIPTION FACTOR 1), also called HEAT-SHOCK FACTOR 1, is a protein that in humans is encoded by the HSF1 gene. The product of HSF1 gene is a heat-shock transcription factor. The International Radiation Hybrid Mapping Consortium maps the HSF1 gene to chromosome 8. HSF1 exists as an inactive monomer in a complex with Hsp40/Hsp70 and Hsp90. Upon stress, such as elevated temperature, HSF1 is released from the chaperone complex and trimerizes. HSF1 is then transported into the nucleus where it is hyperphosphorylated and binds to DNA containing heat shock elements. HSF1's target genes include major inducible heat shock proteins such as Hsp72, and interestingly, noncoding RNA within Satellite III repeat regions. In a novel in vitro system human HSF1 can be activated by nonnative protein, heat, and geldanamycin. HSR1 is constitutively expressed in human and rodent cells and its homologs are functionally interchangeable. Furthermore, Hsf1-deficient mice had a longer free-running period than wildtype littermates.

Molekulargewicht: 80 kDa

Gen-ID: 3297

UniProt: Q00613