GPX3 Antikörper (C-Term)

Produktnummer ABIN7602892

Der Kaninchen Polyklonal Anti-GPX3-Antikörper wurde für WB validiert. Er ist geeignet, GPX3 in Proben von Maus und Ratte zu detektieren.

Kurzübersicht für GPX3 Antikörper (C-Term) (ABIN7602892)

Target: GPX3 (Glutathione Peroxidase 3 (Plasma) (GPX3))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPX3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-GPX3 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-GPX3 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-GPX3 Antibody Picoband® (ABIN7602892). Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GPX3. Human GPX3 shares 100% amino acid (aa) sequence identity with both mouse and rat GPX3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
1. Chambers, I., Frampton, J., Goldfarb, P., Affara, N., McBain, W., Harrison, P. R. The structure of the mouse glutathione peroxidase gene: the selenocysteine in the active site is encoded by the 'termination' codon, TGA. EMBO J. 5: 1221-1227, 1986. 2. Chu, F.-F. The human glutathione peroxidase genes GPX2, GPX3, and GPX4 map to chromosomes 14, 5, and 19, respectively. Cytogenet. Cell Genet. 66: 96-98, 1994. 3. Chu, F.-F., Esworthy, R. S., Doroshow, J. H., Doan, K., Liu, X.-F. Expression of glutathione peroxidase in human liver in addition to kidney, heart, lung, and breast in humans and rodents. Blood 79: 3233-3238, 1992.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu GPX3

Target: GPX3 (Glutathione Peroxidase 3 (Plasma) (GPX3))

Andere Bezeichnung: GPX3

Hintergrund:

Synonyms: GPX3, GPXP, Glutathione peroxidase 3, GPx-3, GSHPx-3, EC 1.11.1.9, Extracellular glutathione peroxidase, Plasma glutathione peroxidase, GPx-P, GSHPx-P

Background: The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is secreted, and is abundantly found in plasma. Downregulation of expression of this gene by promoter hypermethylation has been observed in a wide spectrum of human malignancies, including thyroid cancer, hepatocellular carcinoma and chronic myeloid leukemia. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.

Molekulargewicht: 26 kDa

Gen-ID: 2878

UniProt: P22352

Pathways: Thyroid Hormone Synthesis