ARL1 Antikörper (C-Term)

Produktnummer ABIN7602848

Dieses Anti-ARL1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ARL1 in WB, IHC, IF und FACS. Geeignet für Human.

Kurzübersicht für ARL1 Antikörper (C-Term) (ABIN7602848)

Target: ARL1 (ADP Ribosylation Factor Like GTPase 1 (ARL1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ARL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-ARL1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-ARL1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-ARL1 Antibody Picoband® (ABIN7602848). Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ARL1, identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/mL/1x10^6 cells, Human
1. Gross, M. B. Personal Communication. Baltimore, Md. 7/25/2012. 2. Hong, J.-X., Lee, F.-J. S., Patton, W. A., Lin, C. Y., Moss, J., Vaughan, M. Phospholipid- and GTP-dependent activation of cholera toxin and phospholipase D by human ADP-ribosylation factor-like protein 1 (HARL1). J. Biol. Chem. 273: 15872-15876, 1998. 3. Lu, L., Tai, G., Hong, W. Autoantigen golgin-97, an effector of Arl1 GTPase, participates in traffic from the endosome to the trans-Golgi network. Molec. Biol. Cell 15: 4426-4443, 2004.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu ARL1

Target: ARL1 (ADP Ribosylation Factor Like GTPase 1 (ARL1))

Andere Bezeichnung: ARL1

Hintergrund:

Synonyms: ATP-dependent RNA helicase DDX1, DEAD box protein 1, DEAD box protein retinoblastoma, DBP-RB, DDX1

Tissue Specificity: Highest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord.

Background: ADP-ribosylation factor-like protein 1 is a protein that in humans is encoded by the ARL1 gene. The protein encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molekulargewicht: 20 kDa

Gen-ID: 400

UniProt: P40616