Claudin 3 Antikörper (C-Term)

Produktnummer ABIN7602817

Der Kaninchen Polyklonal Anti-Claudin 3-Antikörper wurde für WB, IF und ICC validiert. Er ist geeignet, Claudin 3 in Proben von Maus und Ratte zu detektieren.

Kurzübersicht für Claudin 3 Antikörper (C-Term) (ABIN7602817)

Target: Claudin 3 (CLDN3)

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Claudin 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-Claudin 3 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-Claudin 3/CLDN3 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Claudin 3/CLDN3 Antibody Picoband® (ABIN7602817). Tested in IF, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Claudin 3/CLDN3, which shares 73.3% and 94.1% amino acid (aa) sequence identity with human and rat Claudin 3/CLDN3, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Mouse
1. Coyne, C. B., Gambling, T. M., Boucher, R. C., Carson, J. L., Johnson, L. G. Role of claudin interactions in airway tight junctional permeability. Am. J. Physiol. Lung Cell. Molec. Physiol. 285: L1166-L1178, 2003. 2. Dube, E., Chan, P. T. K., Hermo, L., Cyr, D. G. Gene expression profiling and its relevance to the blood-epididymal barrier in the human epididymis. Biol. Reprod. 76: 1034-1044, 2007. 3. Furuse, M., Fujita, K., Hiiragi, T., Fujimoto, K., Tsukita, S. Claudin-1 and -2: novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin. J. Cell Biol. 141: 1539-1550, 1998

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Claudin 3

Target: Claudin 3 (CLDN3)

Andere Bezeichnung: Cldn3

Hintergrund:

Synonyms: "Long-chain-fatty-acid--CoA ligase 4, Long-chain acyl-CoA synthetase 4, LACS 4, ACSL4, ACS4, FACL4, LACS4 "

Tissue Specificity: Expressed in the prostate gland and prostate cancers.

Background: Claudin 3, also known as CLDN3, is a protein which in humans is encoded by the CLDN3 gene. This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is a low-affinity receptor for clostridium perfringens enterotoxin (CPE) produced by the bacterium Clostridium perfringens, and the interaction with CPE results in increased membrane permeability by forming small pores in plasma membrane. This protein is highly overexpressed in uterine carcinosarcoma. This protein is also predominantly present in brain endothelial cells, where it plays a specific role in the establishment and maintenance of blood brain barrier tight junction morphology.

Molekulargewicht: 23 kDa

Gen-ID: 12739

UniProt: Q9Z0G9

Pathways: Hepatitis C