CHD2 Antikörper (C-Term)

Produktnummer ABIN7602759

Produktdetails anti-CHD2 Antikörper

Target: CHD2 (Chromodomain Helicase DNA Binding Protein 2 (CHD2))

Bindungsspezifität: C-Term

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Verwendungszweck: Anti-CHD2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-CHD2 Antibody Picoband® (ABIN7602759). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CHD2, which shares 81.8% amino acid (aa) sequence identity with both mouse and rat CHD2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Monkey
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Carvill, G. L., Heavin, S. B., Yendle, S. C., McMahon, J. M., O'Roak, B. J., Cook, J., Khan, A., Dorschner, M. O., Weaver, M., Calvert, S., Malone, S., Wallace, G., and 22 others. Targeted resequencing in epileptic encephalopathies identifies de novo mutations in CHD2 and SYNGAP1. Nature Genet. 45: 825-830, 2013. 2. Kim, Y., Khoshkhoo, S., Frankowski, J. C., Zhu, B., Abbasi, S., Lee, S., Wu, Y. E., Hunt, R. F. Chd2 is necessary for neural circuit development and long-term memory. Neuron 100: 1180-1193, 2018. 3. Kulkarni, S., Nagarajan, P., Wall, J., Donovan, D. J., Donell, R. L., Ligon, A. H., Venkatachalam, S., Quade, B. J. Disruption of chromodomain helicase DNA binding protein 2 (CHD2) causes scoliosis. Am. J. Med. Genet. 146A: 1117-1127, 2008.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu CHD2

Target: CHD2 (Chromodomain Helicase DNA Binding Protein 2 (CHD2))

Andere Bezeichnung: CHD2 (CHD2 Produkte)

Hintergrund:

Synonyms: Chromodomain-helicase-DNA-binding protein 2 (EC:3.6.4.12), CHD-2, ATP-dependent helicase CHD2, CHD2

Tissue Specificity: Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Expressed in sweat glands and bronchiolar epithelium (PubMed:26911677).

Background: Chromodomain-helicase-DNA-binding protein 2 is an enzyme that in humans is encoded by the CHD2 gene. The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. CHD2 catalyzes the assembly of chromatin into periodic arrays, and the N-terminal region of CHD2, which contains tandem chromodomains, serves an auto-inhibitory role in both the DNA-binding and ATPase activities of CHD2.

Molekulargewicht: 250 kDa

Gen-ID: 1106

UniProt: O14647