Nerve Growth Factor Antikörper (C-Term)

Produktnummer ABIN7602750

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Nerve Growth Factor in WB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Nerve Growth Factor Antikörper (C-Term) (ABIN7602750)

Target: Nerve Growth Factor (NGF)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nerve Growth Factor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Nerve Growth Factor Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-NGF Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NGF Antibody Picoband® (ABIN7602750). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human NGF, which shares 91.3% amino acid (aa) sequence identity with both mouse and rat NGF.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
1. Breakefield, X. O., Orloff, G., Castiglione, C., Coussens, L., Axelrod, F. B., Ullrich, A. Structural gene for beta-nerve growth factor not defective in familial dysautonomia. Proc. Nat. Acad. Sci. 81: 4213-4216, 1984. 2. Carrier, A., Rosier, M.-F., Guillemot, F., Goguel, A.-F., Pulcini, F., Bernheim, A., Auffray, C., Devignes, M.-D. Integrated physical, genetic, and genic map covering 3 Mb around the human NGF gene (NGFB) at 1p13. Genomics 31: 80-89, 1996. 3. Carvalho, O. P., Thornton, G. K., Hertecant, J., Houlden, H., Nicholas, A. K., Cox, J. J., Rielly, M., Al-Gazali, L., Woods, C. G. A novel NGF mutation clarifies the molecular mechanism and extends the phenotypic spectrum of the HSAN5 neuropathy. J. Med. Genet. 48: 131-135, 2011.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Andere Bezeichnung: NGF

Hintergrund:

Synonyms: Beta-nerve growth factor, Beta-NGF, NGF, NGFB

Tissue Specificity: Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure. Isoforms IA are widely expressed but not detected in liver nor pancreas, neural expression is strongest in corpus callosum. Isoforms IB are expressed mostly in muscle, cerebellum, placenta and thymus, neural expression in fetal and adult brain, strongest in corpus callosum.

Background: Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. It is mapped to 1p13.2. This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis.

Molekulargewicht: 27 kDa

Gen-ID: 4803

UniProt: P01138

Pathways: Regulation of Cell Size