Nebulette Antikörper (AA 86-842)

Produktnummer ABIN7602577

Der Kaninchen Polyklonal Anti-Nebulette-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, Nebulette in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Nebulette Antikörper (AA 86-842) (ABIN7602577)

Target: Nebulette (NEBL)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nebulette Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-Nebulette Antikörper

Bindungsspezifität: AA 86-842

Verwendungszweck: Anti-NEBL Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NEBL Antibody Picoband® (ABIN7602577). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NEBL recombinant protein (Position: E86-K842).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Arimura, T., Nakamura, T., Hiroi, S., Satoh, M., Takahashi, M., Ohbuchi, N., Ueda, K., Nouchi, T., Yamaguchi, N., Akai, J., Matsumori, A., Sasayama, S., Kimura, A. Characterization of the human nebulette gene: a polymorphism in an actin-binding motif is associated with nonfamilial idiopathic dilated cardiomyopathy. Hum. Genet. 107: 440-451, 2000. 2. Li, B., Zhuang, L., Trueb, B. Zyxin interacts with the SH3 domains of the cytoskeletal proteins LIM-nebulette and Lasp-1. J. Biol. Chem. 279: 20401-20410, 2004. 3. Millevoi, S., Trombitas, K., Kolmerer, B., Kostin, S., Schaper, J., Pelin, K., Granzier, H., Labeit, S. Characterization of nebulette and nebulin and emerging concepts of their roles for vertebrate Z-discs. J. Molec. Biol. 282: 111-123, 1998.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu Nebulette

Target: Nebulette (NEBL)

Andere Bezeichnung: NEBL

Hintergrund:

Synonyms: Protein phosphatase 1E, Ca (2+)/calmodulin-dependent protein kinase phosphatase N, CaMKP-N, CaMKP-nucleus, CaMKN, Partner of PIX 1, Partner of PIX-alpha, Partner of PIXA, PPM1E, CAMKN, KIAA1072, POPX1

Tissue Specificity: Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts.

Background: Nebulette is a cardiac-specific isoform belonging to the nebulin family of proteins. It is encoded by the NEBL gene. This gene encodes a nebulin like protein that is abundantly expressed in cardiac muscle. The encoded protein binds actin and interacts with thin filaments and Z-line associated proteins in striated muscle. This protein may be involved in cardiac myofibril assembly. A shorter isoform of this protein termed LIM nebulette is expressed in non-muscle cells and may function as a component of focal adhesion complexes. Alternate splicing results in multiple transcript variants.

Molekulargewicht: 126 kDa

Gen-ID: 10529

UniProt: O76041