PYHIN1 Antikörper (AA 86-488)

Produktnummer ABIN7602574

Produktdetails anti-PYHIN1 Antikörper

Target: PYHIN1 (Pyrin and HIN Domain Family, Member 1 (PYHIN1))

Bindungsspezifität: AA 86-488

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PYHIN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Verwendungszweck: Anti-PYHIN1 Antibody Picoband®

Produktmerkmale: Anti-PYHIN1 Antibody Picoband® (ABIN7602574). Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human PYHIN1 recombinant protein (Position: K86-H488). Human PYHIN1 shares 45.6% amino acid (aa) sequence identity with mouse PYHIN1.

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ding, Y., Lee, J.-F., Lu, H., Lee, M.-H., Yan, D.-H. Interferon-inducible protein IFIX-alpha-1 functions as a negative regulator of HDM2. Molec. Cell. Biol. 26: 1979-1996, 2006. 2. Ding, Y., Wang, L., Su, L.-K., Frey, J. A., Shao, R., Hunt, K. K., Yan, D.-H. Antitumor activity of IFIX, a novel interferon-inducible HIN-200 gene, in breast cancer. Oncogene 23: 4556-4566, 2004. 3. Yamaguchi, H., Ding, Y., Lee, J.-F., Zhang, M., Pal, A., Bornmann, W., Yan, D.-H., Hung, M.-C. Interferon-inducible protein IFIX-alpha inhibits cell invasion by upregulating the metastasis suppressor maspin. Molec. Carcinogen. 47: 739-743, 2008.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu PYHIN1

Target: PYHIN1 (Pyrin and HIN Domain Family, Member 1 (PYHIN1))

Andere Bezeichnung: PYHIN1 (PYHIN1 Produkte)

Hintergrund: The protein encoded by this gene belongs to the HIN-200 family of interferon-inducible proteins that share a 200-amino acid signature motif at their C-termini. HIN200 proteins are primarily nuclear and are involved in transcriptional regulation of genes important for cell cycle control, differentiation, and apoptosis. Downregulation of this gene is associated with breast cancer. This protein acts as a tumor suppressor by promoting ubiquitination and subsequent degradation of MDM2, which leads to stabilization of p53/TP53. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Molekulargewicht: 55 kDa

Gen-ID: 149628

UniProt: Q6K0P9