MED12 Antikörper (AA 752-1974)

Produktnummer ABIN7602421

Der Kaninchen Polyklonal Anti-MED12-Antikörper wurde für ELISA und WB validiert. Er ist geeignet, MED12 in Proben von Human zu detektieren.

Kurzübersicht für MED12 Antikörper (AA 752-1974) (ABIN7602421)

Target: MED12 (Mediator Complex Subunit 12 (MED12))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MED12 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Produktdetails anti-MED12 Antikörper

Bindungsspezifität: AA 752-1974

Verwendungszweck: Anti-MED12 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-MED12 Antibody Picoband® (ABIN7602421). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human MED12 recombinant protein (Position: Q752-Q1974).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Barbieri, C. E., Baca, S. C., Lawrence, M. S., Demichelis, F., Blattner, M., Theurillat, J.-P., White, T. A., Stojanov, P., Van Allen, E., Stransky, N., Nickerson, E., Chae, S.-S., and 34 others. Exome sequencing identifies recurrent SPOP, FOXA1 and MED12 mutations in prostate cancer. Nature Genet. 44: 685-689, 2012. 2. Beyer, K. S., Klauck, S. M., Benner, A., Poustka, F., Poustka, A. Association studies of the HOPA dodecamer duplication variant in different subtypes of autism. Am. J. Med. Genet. 114: 110-115, 2002. 3. Cools, F., Jaeken, J. Hardikar syndrome: a new syndrome with cleft lip/palate, pigmentary retinopathy and cholestasis. Am. J. Med. Genet. 71: 472-474, 1997.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu MED12

Target: MED12 (Mediator Complex Subunit 12 (MED12))

Andere Bezeichnung: MED12

Hintergrund:

Synonyms: Protein argonaute-1,Argonaute1,hAgo1,Argonaute RISC catalytic component 1,Eukaryotic translation initiation factor 2C 1,eIF-2C 1,eIF2C 1,Putative RNA-binding protein Q99,AGO1,EIF2C1,

Tissue Specificity: Detected in blood plasma (at protein level).

Background: Mediator of RNA polymerase II transcription, subunit 12 homolog (S. cerevisiae), also known as MED12, is a human gene found on the X chromosome. The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome.

Molekulargewicht: 260 kDa

Gen-ID: 9968

UniProt: Q93074

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Tube Formation