Alanine--tRNA Ligase, Mitochondrial (AARS-1) (AA 729-968) Antikörper

Produktnummer ABIN7602380

Dieses Anti--Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von in ELISA, IF, WB, FACS und ICC. Geeignet für Human.

Kurzübersicht für Alanine--tRNA Ligase, Mitochondrial (AARS-1) (AA 729-968) Antikörper (ABIN7602380)

Target: Alanine--tRNA Ligase, Mitochondrial (AARS-1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: ELISA, Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails

Bindungsspezifität: AA 729-968

Verwendungszweck: Anti-AlaRS/AARS1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-AlaRS/AARS1 Antibody Picoband® (ABIN7602380). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human AlaRS/AARS1 recombinant protein (Position: R729-N968).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chihade, J. W., Brown, J. R., Schimmel, P. R., Ribas de Pouplana, L. Origin of mitochondria in relation to evolutionary history of eukaryotic alanyl-tRNA synthetase. Proc. Nat. Acad. Sci. 97: 12153-12157, 2000. 2. Guo, M., Chong, Y. E., Beebe, K., Shapiro, R., Yang, X.-L., Schimmel, P. The C-Ala domain brings together editing and aminoacylation functions on one tRNA. Science 325: 744-747, 2009. Note: Erratum: Science 326: 46 only, 2009. 3. Guo, M., Chong, Y. E., Shapiro, R., Beebe, K., Yang, X.-L., Schimmel, P. Paradox of mistranslation of serine for alanine caused by AlaRS recognition dilemma. Nature 462: 808-812, 2009.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Antigendetails

Target: Alanine--tRNA Ligase, Mitochondrial (AARS-1)

Andere Bezeichnung: AARS1

Hintergrund:

Synonyms: Fos-related antigen 1, FRA-1, FOSL1, FRA1

Tissue Specificity: Adipocytes.

Background: An aminoacyl-tRNA synthetase (aaRS or ARS), also called tRNA-ligase, is an enzyme that attaches the appropriate amino acid onto its corresponding tRNA. The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41 % identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.

Molekulargewicht: 107 kDa

Gen-ID: 16

UniProt: P49588