Occludin Antikörper (AA 7-367)

Produktnummer ABIN7602327

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Occludin in WB und ELISA. Er zeigt eine Reaktivität gegenüber Maus und Ratte.

Kurzübersicht für Occludin Antikörper (AA 7-367) (ABIN7602327)

Target: Occludin (OCLN)

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Occludin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Occludin Antikörper

Bindungsspezifität: AA 7-367

Verwendungszweck: Anti-Occludin/Ocln Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Occludin/Ocln Antibody Picoband® (ABIN7602327). Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Occludin/Ocln recombinant protein (Position: E7-Y367).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Ando-Akatsuka, Y., Saitou, M., Hirase, T., Kishi, M., Sakakibara, A., Itoh, M., Yonemura, S., Furuse, M., Tsukita, S. Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues. J. Cell Biol. 133: 43-47, 1996. 2. Argaw, A. T., Gurfein, B. T., Zhang, Y., Zameer, A., John, G. R. VEGF-mediated disruption of endothelial CLN-5 promotes blood-brain barrier breakdown. Proc. Nat. Acad. Sci. 106: 1977-1982, 2009. 3. Barrios-Rodiles, M., Brown, K. R., Ozdamar, B., Bose, R., Liu, Z., Donovan, R. S., Shinjo, F., Liu, Y., Dembowy, J., Taylor, I. W., Luga, V., Przulj, N., Robinson, M., Suzuki, H., Hayashizaki, Y., Jurisica, I., Wrana, J. L. High-throughput mapping of a dynamic signaling network in mammalian cells. Science 307: 1621-1625, 2005.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu Occludin

Target: Occludin (OCLN)

Andere Bezeichnung: Ocln

Hintergrund:

Synonyms: Cyclin-dependent kinase inhibitor 1C, Cyclin-dependent kinase inhibitor p57, p57Kip2, CDKN1C, KIP2

Tissue Specificity: Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. Expressed in the eye. High levels are seen in the placenta while low levels are seen in the liver.

Background: Occludin is an enzyme (EC 1.6) that oxidizes NADH. Predicted to enable protein domain specific binding activity. Involved in negative regulation of gene expression and tight junction organization. Located in several cellular components, including apical plasma membrane, apicolateral plasma membrane, and bicellular tight junction. Is expressed in several structures, including alimentary system, brain, genitourinary system, respiratory system, and sensory organ. Human ortholog(s) of this gene implicated in Crohn's disease and pseudo-TORCH syndrome 1. Orthologous to human OCLN (occludin).

Molekulargewicht: 59 kDa

Gen-ID: 18260

UniProt: Q61146

Pathways: Cell-Cell Junction Organization, Hepatitis C