DGKZ Antikörper (AA 664-928)

Produktnummer ABIN7602261

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch DGKZ in WB, IHC, ELISA und FACS. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus.

Kurzübersicht für DGKZ Antikörper (AA 664-928) (ABIN7602261)

Target: DGKZ (Diacylglycerol Kinase, zeta (DGKZ))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DGKZ Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Produktdetails anti-DGKZ Antikörper

Bindungsspezifität: AA 664-928

Verwendungszweck: Anti-DGKZ/DGK-zeta Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-DGKZ/DGK-zeta Antibody Picoband® (ABIN7602261). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human DGKZ/DGK-zeta recombinant protein (Position: E664-V928).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bunting, M., Tang, W., Zimmerman, G. A., McIntyre, T. M., Prescott, S. M. Molecular cloning and characterization of a novel human diacylglycerol kinase. J. Biol. Chem. 271: 10230-10236, 1996. 2. Gross, M. B. Personal Communication. Baltimore, Md. 4/11/2014. 3. Topham, M. K., Bunting, M., Zimmerman, G. A., McIntyre, T. M., Blackshear, P. J., Prescott, S. M. Protein kinase C regulates the nuclear localization of diacylglycerol kinase-zeta. Nature 394: 697-700, 1998.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu DGKZ

Target: DGKZ (Diacylglycerol Kinase, zeta (DGKZ))

Andere Bezeichnung: DGKZ

Hintergrund:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 21, Deubiquitinating enzyme 21, Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, USP21, USP23, PP1490

Tissue Specificity: Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung.

Background: Diacylglycerol kinase zeta is an enzyme that in humans is encoded by the DGKZ gene. The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified.

Molekulargewicht: 124 kDa

Gen-ID: 8525

UniProt: Q13574

Pathways: Myometrial Relaxation and Contraction