PHKG1 Antikörper (AA 62-353)

Produktnummer ABIN7602190

Der Kaninchen Polyklonal Anti-PHKG1-Antikörper wurde für WB, ELISA und FACS validiert. Er ist geeignet, PHKG1 in Proben von Human zu detektieren.

Kurzübersicht für PHKG1 Antikörper (AA 62-353) (ABIN7602190)

Target: PHKG1 (phosphorylase Kinase, gamma 1 (Muscle) (PHKG1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PHKG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-PHKG1 Antikörper

Bindungsspezifität: AA 62-353

Verwendungszweck: Anti-PHKG1 Antibody Picoband®

Produktmerkmale: Anti-PHKG1 Antibody Picoband® (ABIN7602190). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human PHKG1 recombinant protein (Position: E62-R353). Human PHKG1 shares 93.8% and 94.2% amino acid (aa) sequence identity with mouse and rat PHKG1, respectively.

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Burwinkel, B., Hu, B., Schroers, A., Clemens, P. R., Moses, S. W., Shin, Y. S., Pongratz, D., Vorgerd, M., Kilimann, M. W. Muscle glycogenosis with low phosphorylase kinase activity: mutations in PHKA1, PHKG1 or six other candidate genes explain only a minority of cases. Europ. J. Hum. Genet. 11: 516-526, 2003. 2. Chamberlain, J. S., vanTuinen, P., Reeves, A. A., Philip, B. A., Caskey, C. T. Isolation of cDNA clones for the catalytic gamma subunit of mouse muscle phosphorylase kinase: expression of mRNA in normal and mutant Phk mice. Proc. Nat. Acad. Sci. 84: 2886-2890, 1987. 3. Jones, T. A., da Cruz e Silva, E. F., Spurr, N. K., Sheer, D., Cohen, P. T. Localization of the gene encoding the catalytic gamma subunit of phosphorylase kinase to human chromosome bands 7p12-q21. Biochim. Biophys. Acta 1048: 24-29, 1990.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu PHKG1

Target: PHKG1 (phosphorylase Kinase, gamma 1 (Muscle) (PHKG1))

Andere Bezeichnung: PHKG1

Hintergrund: Phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform is an enzyme that in humans is encoded by the PHKG1 gene. This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12. Multiple transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 40 kDa

Gen-ID: 5260

UniProt: Q16816

Pathways: Cellular Glucan Metabolic Process