SUB1 Antikörper (AA 62-127)

Produktnummer ABIN7602185

Dieser Anti-SUB1 Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von SUB1 in WB, IHC, IF und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für SUB1 Antikörper (AA 62-127) (ABIN7602185)

Target: SUB1 (Activated RNA Polymerase II Transcriptional Coactivator p15 (SUB1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SUB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: 8D9D1

Produktdetails anti-SUB1 Antikörper

Bindungsspezifität: AA 62-127

Verwendungszweck: Anti-PC4/SUB1 Antibody Picoband® (monoclonal, 8D9D1)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-PC4/SUB1 Antibody Picoband® (monoclonal, 8D9D1) (ABIN7602185). Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human PC4/SUB1 recombinant protein (Position: N62-L127).

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene SUB1: SUB1 homolog (S. cerevisiae)". 2. Kretzschmar M, Kaiser K, Lottspeich F, Meisterernst M (August 1994). "A novel mediator of class II gene transcription with homology to viral immediate-early transcriptional regulators". Cell 78 (3): 525-34. 3. Ge H, Roeder RG (August 1994). "Purification, cloning, and characterization of a human coactivator, PC4, that mediates transcriptional activation of class II genes". Cell 78 (3): 513-23.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu SUB1

Target: SUB1 (Activated RNA Polymerase II Transcriptional Coactivator p15 (SUB1))

Andere Bezeichnung: SUB1

Hintergrund:

Synonyms: Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein, ACTN3

Tissue Specificity: Expressed only in a subset of type 2 skeletal muscle fibers.

Background: Activated RNA polymerase II transcriptional coactivator p15, also known as positive cofactor 4 (PC4) or SUB1 homolog, is a protein that in humans is encoded by the SUB1 gene. This gene is mapped to 5p13.3. The transcriptional cofactor PC4 is an ancient single-strand DNA (ssDNA)-binding protein that has a homologue in bacteriophage T5 where it is likely the elusive replicative ssDNA-binding protein. The recombinant PC4 is shown to function identically to the native protein through its interaction with TAFs.

Molekulargewicht: 19 kDa

Gen-ID: 10923

UniProt: P53999