Amphiphysin Antikörper (AA 58-683)

Produktnummer ABIN7602082

Der Kaninchen Polyklonal Anti-Amphiphysin-Antikörper wurde für WB, ELISA und FACS validiert. Er ist geeignet, Amphiphysin in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Amphiphysin Antikörper (AA 58-683) (ABIN7602082)

Target: Amphiphysin (AMPH)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Amphiphysin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-Amphiphysin Antikörper

Bindungsspezifität: AA 58-683

Verwendungszweck: Anti-Amphiphysin/AMPH Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Amphiphysin/AMPH Antibody Picoband® (ABIN7602082). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Amphiphysin/AMPH recombinant protein (Position: R58-K683).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. David, C., Solimena, M., De Camilli, P. Autoimmunity in stiff-man syndrome with breast cancer is targeted to the C-terminal region of human amphiphysin, a protein similar to the yeast proteins, Rvs167 and Rvs161. FEBS Lett. 351: 73-79, 1994. 2. De Camilli, P., Thomas, A., Cofiell, R., Folli, F., Lichte, B., Piccolo, G., Meinck, H.-M., Austoni, M., Fassetta, G., Bottazzo, G., Bates, D., Cartlidge, N., Solimena, M., Kilimann, M. W. The synaptic vesicle-associated protein amphiphysin is the 128-kD autoantigen of stiff-man syndrome with breast cancer. J. Exp. Med. 178: 2219-2223, 1993. 3. Di Paolo, G., Sankaranarayanan, S., Wenk, M. R., Daniell, L., Perucco, E., Caldarone, B. J., Flavell, R., Picciotto, M. R., Ryan, T. A., Cremona, O., De Camilli, P. Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice. Neuron 33: 789-804, 2002.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu Amphiphysin

Target: Amphiphysin (AMPH)

Andere Bezeichnung: AMPH

Hintergrund:

Synonyms: Neuronal acetylcholine receptor subunit alpha-5,CHRNA5,NACHRA5,

Background: Amphiphysin is a protein that in humans is encoded by the AMPH gene. This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.

Molekulargewicht: 115-125 kDa

Gen-ID: 273

UniProt: P49418