GAR1 Antikörper (AA 58-165)

Produktnummer ABIN7602076

Der Kaninchen Polyklonal Anti-GAR1-Antikörper wurde für ELISA, WB, IHC, IF, ICC und FACS validiert. Er ist geeignet, GAR1 in Proben von Human zu detektieren.

Kurzübersicht für GAR1 Antikörper (AA 58-165) (ABIN7602076)

Target: GAR1 (GAR1 Ribonucleoprotein Homolog (Yeast) (GAR1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GAR1 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-GAR1 Antikörper

Bindungsspezifität: AA 58-165

Verwendungszweck: Anti-NOLA1/GAR1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NOLA1/GAR1 Antibody Picoband® (ABIN7602076). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NOLA1/GAR1 recombinant protein (Position: F58-K165).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Dragon, F., Pogacic, V., Filipowicz, W. In vitro assembly of human H/ACA small nucleolar RNPs reveals unique features of U17 and telomerase RNAs. Molec. Cell. Biol. 20: 3037-3048, 2000. 2. Tollervey, D., Kiss, T. Function and synthesis of small nucleolar RNAs. Curr. Opin. Cell Biol. 9: 337-342, 1997.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu GAR1

Target: GAR1 (GAR1 Ribonucleoprotein Homolog (Yeast) (GAR1))

Andere Bezeichnung: GAR1

Hintergrund:

Synonyms: Forkhead box protein F1, Forkhead-related activator 1, FREAC-1, Forkhead-related protein FKHL5, Forkhead-related transcription factor 1, FOXF1, FKHL5, FREAC1

Tissue Specificity: Expressed in kidney.

Background: H/ACA ribonucleoprotein complex subunit 1 is a protein that in humans is encoded by the GAR1 gene. This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA2 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The encoded protein of this gene contains two glycine- and arginine-rich domains and is related to Saccharomyces cerevisiae Gar1p. Two splice variants have been found for this gene.

Molekulargewicht: 25 kDa

Gen-ID: 54433